Poziv na predavanje izv. prof. Victora Weissa

Objavljeno 14.11.2022.

Pozivam vas na predavanje pod naslovom “Nano electrospray gas-phase electrophoretic mobility molecular analysis (nES GEMMA) based molecular weight determination in the nm range“, koje će održati izv. prof. Victor Weiss, gost sa Tehničkog sveučilišta u Beču (TU Wien), u srijedu 16.11.2022. u 13:30 u predavaonici krila III IRB-a.

Izvanredni prof. Mag. Dr. Victor Weiss zaposlen je na TU Wien, Institute for Chemical Technologies and Analytics u Beču. Stručnjak je za analizu i elektroforetsku separaciju virusa i drugih nanočestica, te za povezivanje elektroforetske separacije s analitičkim metodama, poput spektrometrije masa. Dobitnik je nekoliko nagrada za znanstvena postignuća i autor je 44 znanstvena rada.

Sažetak predavanja možete pronaći u nastavku.

“Nano electrospray gas-phase electrophoretic mobility molecular analysis (nES GEMMA) based molecular weight determination in the nm range Assoc. Prof. Victor Weiss, TU Wien Gas-phase electrophoresis on a nano electrospray gas-phase electrophoretic mobility molecular analyzer (nES GEMMA also known as nES differential mobility analyzer, nES DMA) separates surface dry, single-charged particles according to their electrophoretic mobility diameter (EMD) in a high laminar sheath flow of particle-free, ambient air. Transfer of analytes from a volatile electrolyte solution to the gas-phase prior gas-phase electrophoresis occurs via a nES process. Obtained droplets are dried and concomitantly charge equilibration occurs in a bipolar atmosphere induced e.g. by a 210Po α-particle source. Alternatively, a soft X-Ray charger or a corona discharge can be employed. The determined EMD corresponds to the size of spherical nanoparticles. Gas-phase electrophoresis was demonstrated as soft ionization / analysis method in the past even enabling targeting of non-covalent complexes. In addition, as shown for proteins, the EMD also relates to their molecular weight (MW) enabling to calculate an analyte MW based on its EMD. Focusing on viruses, virus-like particles (VLPs) and polysaccharides the deviation of their EMD / MW correlation from the protein case will be demonstrated. Furthermore, gas-phase electrophoresis will be compared to mass spectrometry, demonstrating the assets and drawbacks of both methods. Based on obtained correlations and nES GEMMA based EMD vales, MW values for analytes of unknown MW can be calculated given that the substance class (protein, virus, VLP, polysaccharide,…) of the analyte of question is known.“