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PovratakObjavljeno 03.07.2023.
Hrvatsko društvo za biokemiju i molekularnu biologiju i Kemijski odsjek Prirodoslovno-matematičkog fakulteta Sveučilišta u Zagrebu organiziraju zajednički znanstveni kolokvij na kojem će dr. sc. Silvija Bilokapić (Department of Structural Biology, St. Jude Children’s Research Hospital, Memphis, USA) održati predavanje pod naslovom “Chromatin localization of nucleophosmin organizes ribosome biogenesis”.
Predavanje će se održati u ponedjeljak 10. srpnja u 14:00 sati u predavaonici P1 (prizemlje zgrade Kemijskog odsjeka PMF-a, Horvatovac 102a). Predavanje će se moći pratiti i online preko sljedeće poveznice https://zoom.us/j/94005450567?pwd=RjlzQ0NPa0lzQ2M3eXg0eU94R2M0dz09
Sažetak:
Chromatin localization of nucleophosmin organizes ribosome biogenesis Ribosome biogenesis takes place in the nucleolus, a nuclear membrane-less organelle. Although well studied, it remains unknown how nascent ribosomal subunits separate from the central chromatin compartment and move to the outer granular component, where maturation occurs. We find that the Schizosaccharomyces pombe nucleophosmin-like protein Fkbp39 localizes to rDNA sites encoding the 60S subunit rRNA, and this localization contributes to its specific association with nascent 60S subunits. Fkbp39 dissociates from chromatin to bind nascent 60S subunits, causing the latter to partition away from chromatin and from nascent 40S subunits through liquid-liquid phase separation. In vivo, Fkbp39 binding directs the translocation of nascent 60S subunits toward the nucleophosmin-rich granular component. This process increases the efficiency of 60S subunit assembly, facilitating the incorporation of 60S RNA domain III. Thus, chromatin localization determines the specificity of nucleophosmin in sorting nascent ribosomal subunits and coordinates their movement into specialized assembly compartments within the nucleolus.